《植物生理学报》 2012, 48(2): 135-140

低能离子束辐照下水稻中与ABA相关基因的差异表达

李勇慧, 于相丽, 程彦伟, 押辉远*

洛阳师范学院生命科学系, 河南洛阳471022

通信作者：押辉远；E-mail: yahuiyuan@yahoo.com.cn；Tel: 0379-65526176

摘　要：

本文检测了用基因芯片筛选出水稻种子被低能N+辐照后引起的差异表达的ABA代谢和信号途径相关基因。结果显示, 与ABA合成相关的ZDS、Lyc-β、ZEP、NCED、SDR这五种酶的基因表达量均为上调; 受ABA调控的的H+-ATPase、NR、Rubisco的基因表达变化显著; ABA依赖的逆境应答蛋白DREB和ASR的表达量上调; 受ABA信号转导调控的蛋白LEA的表达量下调, GAD和P5CS的表达量上调。这些结果表明, 6×1017 N+·cm-2剂量的辐照可能促进了ABA的合成和幼苗气孔的开放, 同时促进了ABA信号系统并激活或抑制了一些相关基因的表达。

关键词：氮离子束辐照; 水稻; ABA; 基因芯片

收稿：2011-07-25   修定：2011-12-27

资助：国家自然科学基因基金 (30800204)、河南省教育厅自然科学基础研究计划(2009B180019和2010B180020)和洛阳师范学院省部级以上项目培育金(10000858)。

Different Expression of ABA-Related Genes in Rice under Low-Energy N⁺ Irradiation

LI Yong-Hui, YU Xiang-Li, CHENG Yan-Wei, YA Hui-Yuan*

College of Life Sciences, Luoyang Normal University, Luoyang, Henan 471022, China

Corresponding author: YA Hui-Yuan; E-mail: yahuiyuan@yahoo.com.cn; Tel: 0379-65526176

Abstract:

The different expression of ABA metabolism and signaling pathway-related genes in the rice seeds induced by low-energy N+ irradiation were examined using Agilent Rice Oligo Microarray. The results showed that the gene expressions of ZDS, Lyc-β, ZEP, NCED, and SDR which were related with ABA synthesis were up-regulated. The expressions of H+-ATPase, NR, Rubisco which were ABA-regulated functional proteins had significant changes. The expressions of ABA-dependent stress response proteins DREB and ASR were also upregulated. And the expressions of ABA signal-induced protein LEA was down-regulated while GAD and P5CS were up-regulated. These results suggested that the 6×10¹⁷ N+·cm^-2 nitrogen ion beam might promote the ABA synthesis and the seedling stomatal opening, while leading to ABA signaling system and activation or inhibition of gene expression.

Key words: N+ beam irradiation; rice; abscisic acid; gene chip